WSEAS Transactions on Environment and Development

Print ISSN: 1790-5079
E-ISSN: 2224-3496

Volume 13, 2017

Notice: As of 2014 and for the forthcoming years, the publication frequency/periodicity of WSEAS Journals is adapted to the 'continuously updated' model. What this means is that instead of being separated into issues, new papers will be added on a continuous basis, allowing a more regular flow and shorter publication times. The papers will appear in reverse order, therefore the most recent one will be on top.

Volume 13, 2017

Separation and Characterization of Pollutant-Degrading Microbes Separated from Surface Seawater

AUTHORS: Kiwamu Shiiba, Ryotaro Matsui, Yasuyuki Kamata, Tomoko Abe

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ABSTRACT: Microbes capable of degrading pollutant such as 1,4-dioxane were selected from the seawater samples of four coastal areas of Okinawa located in the most southern islands of Japan using the developed method and a seawater-charcoal perfusion apparatus. Specifically, aerobic culture in the apparatus led to the selection of candidate 1,4-dioxane degraders. Among these, strain RM-31 and KY-3 exhibited the strongest 1,4-dioxane degradation ability. 16S rDNA sequencing and the similarity analysis of strain RM-31 and KY-3 suggested that this organism was most closely related to Pseudonocardia carboxydivorans and Rhodococcus erythropolis respectively. These species are similar to Pseudonocardia and Rhodococcus spp. which has previously been reported as a 1,4-dioxane degrader. However, it was probably first bacteria from the seawater that can exert a strong degrading ability. The biodegradation rate of 1,4-dioxane by these strains at 25°C in broth medium with 3% NaCl was almost 20% faster than that without NaCl. PCR amplification test indicated that isolated strains had the SDIMO (soluble di-iron monooxygenase) ɑ-subunit gene.

KEYWORDS: 1,4-dioxane, degrading microbe, seawater-charcoal perfusion apparatus, Psedonocardia, Rhodococcus, hydrophilic interaction chromatography, soluble di-iron monooxygenase


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WSEAS Transactions on Environment and Development, ISSN / E-ISSN: 1790-5079 / 2224-3496, Volume 13, 2017, Art. #35, pp. 346-349

Copyright © 2017 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution License 4.0

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