AUTHORS: Juan Carlos Beltrán-Prieto, Long Huynh Bach Son Nguyen

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ABSTRACT: The process of inhibition of enzymes is important because it serves as a fundamental control mechanism in many biological systems and allows the regulation of metabolic pathways. In fact, many medications act by inhibiting specific enzymes in the brain or in body tissues. Therefore, the understanding of enzymatic inhibition mechanism is, essential. Inhibitors are frequently used as tools for the study of the mechanism of the enzymes themselves. In the present paper we have studied the process of substrate inhibition by developing a mathematical model that allow us to understand the influence of initial amount of substrate and initial biomass concentration on maximum growth rate value. Numerical analysis using Matlab software was performed to model this inhibition process.

KEYWORDS: substrate concentration, inhibition, numerical analysis, simulation, enzyme kinetics, yield, maximum growth rate

REFERENCES:

[1] R. K. Murray, Harper’s Illustrated Biochemistry. McGraw-Hill Medical, 2012.

[2] Y. Taniguchi et al., Quantifying E. coli Proteome and Transcriptome with SingleMolecule Sensitivity in Single Cells, Science Vol. 329, No. 5991, 2010, pp. 533–538.

[3] J. J. Heijnen and R. Kleerebezem, Bioenergetics of Microbial Growth, in Encyclopedia of Industrial Biotechnology, Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010, pp. 1–66.

[4] J. W. Finley and J. M. DeMan, Vitamins, in Principles of Food Chemistry, Springer International Publishing, 2018, pp. 365–395.

[5] K.V. Ramana, J.R. Xavier, and R.K. Sharma, Pharmaceutical. Biotechnology Current Research Vol. 1, No. 1. 2017, pp. 1-10.

[6] M. Lieberman, A. D. Marks, and A. Peet, Marks’ basic medical biochemistry : a clinical approach, Wolters Kluwer Health/Lippincott Williams & Wilkins, 2013.

[7] Y. Chisti and M. Moo-Young, Bioreactors, Encyclopedia of Physical Science and Technology, Academic Press, 2003 pp. 247– 271.

[8] F. Garcia-Ochoa and E. Gomez, Biotechnology Advances. Vol. 27, No. 2, 2009, pp. 153-176.

[9] M. de. Cuyper and J. W. M. Bulte, Physics and chemistry basis of biotechnology. New York: Kluwer Academic Publishers, 2001.

[10] G. D. Najafpour, Biochemical engineering and biotechnology. New York: Elsevier, 2007.

[11] N.S. Panikov, Microbial Growth Kinetics, Springer, Netherlands,1995, pp. 378

[12] K. Kovárová-Kovar and T. Egli, Microbiology and Molecular Biology Reviews, Vol. 62, No. 3, 1998, pp. 646-666.

[13] M. Matsuda and T. Sugawara, Evaluation of Several Mathematical Models for Fitting the Growth and Kinetics of the Catechol-degrading Candida parapsilopsis: Part 2, Journal of Environmental Bioremediation and Toxicology, Vol. 2, No. 2, 2017, pp. 53–57.

[14] J.E. Bailey and D.F. Ollis, Biochemical Engineering Fundamentals, Tata McGraw-Hill, New Delhi, 2010.

[15] Y. Tan, Z.-X. Wang, and K.C. Marshall, Modeling substrate inhibition of microbial growth, Biotechnology and Bioengineering. Vl. 52, No. 5. 2000, pp. 602-608.

[16] M.S.M. Annuar, I.K.P. Tan, S. Ibrahim, and K.B. Ramachandran, A kinetic model for growth and biosynthesis of medium-chainlength poly-(3-hydroxyalkanoates) in

[17] pseudomonas putida, Brazilian Journal of Chemical Engineering, Vol. 25, No. 2, 2008, pp. 217-228.

[18] A.K. Jana, Chemical Process Modelling and Computer Simulation, PHI Learning, New Delhi, 2001, pp 90-116.

[19] D. C. Montgomery, Design and Analysis of Experiments, Mexico: Wiley, 2004.

[20] F.A. Cuevas-Ortiz, M.I. Neria-González, and R. Aguilar-López, Nonlinear observer for switched systems: application to a batch bioreactor Revista Mexicana de Ingeniería Química Vol. 14, No. 1, 2015, pp. 137-147.

[21] R. Sathya, M. Rasi, and L. Rajendran, Nonlinear analysis of Haldane kinetic model in phenol degradation in batch operations Kinetics and Catalysis. Vol. 56, No. 2, 2015, pp. 141- 146.

[22] T. E. Creighton, Encyclopedia of Molecular Biology. John Wiley, 1999.

[23] D. Tazdait, T. Tazdait, N. Abdi, H. Grib, H. Lounici, A. Pauss, N. Mameri, Comparison of different models of substrate inhibition in aerobic batch biodegradation of malathion, Turkish Journal of Engineering & Environmental Sciences, Vol. 2013, No. 37, 2013, pp. 221–230