AUTHORS: Ghadeer A. R. Y. Suaifan, Mohammed Zourob

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ABSTRACT: Paper-based magnetic nanoparticle biosensor for the evaluation of active PSA level was designed. This biosensing configuration is based on the detection of PSA amylodic activity using a specific peptide substrate, sandwiched between magnetic nanonanoparticles and gold sensing platform placed on the top of a paper support. Upon PSA addition, an external magnet fixed at the back of the sensor support would accelerate the cleavage of the magnetic nanoparticles-peptide moieties away from the surface. Colorimetric change from black to golden color was visualized by the naked eye. This biosensor was amenable for a qualitative and quantitative PSA detection in buffer and in spiked urine samples with a lower detection limit of 1 ng/mL and 5 ng/mL, respectively. In conclusion, the designed colorimetric biosensor pinpointed superiority in criteria's including sensitivity; simplicity and fast processing time.

KEYWORDS: Protease, Prostate Cancer, Prostate Specific Antigen, Colorimetric, Biosenser, Magnetic nano-particles

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